Current Research

Evaluation on the in vivo behaviour of HUCPVCs Differentiation of HUCPVCs toward cardiomyocyte-like cells.

Background

Bsc in Applied Biology, University of Minho, Portugal, 2000.

PhD in Materials Science and Engineering – Tissue Engineering, University of Minho, Portugal, 2005.

Awards and Scholarships

• 2000: University of Minho Merit Prize for outstanding student achievements.
• 2001-2004: PhD scholarship from the Portuguese Foundation for Science and Technology.
• 2002: Travelship awarded from Foundation Calouste Gulbenkian to attend the 5th International Meeting of the Tissue Engineering Society International (TESI), held in Kobe, Japan.
• 2005: Honorary mention awarded by The Society for Biomaterials to the work “Starch Based Scaffolds Designed for Bone Tissue Engineering Applications: Cytotoxicity, Cell Culture and In Vivo Evaluation” presented on the 30th Annual Meeting of The Society for Biomaterials, Memphis, Tennessee, USA, April 2005.
• 2005- present: Post-Doctoral fellowship from the Portuguese Foundation for Science and Technology.

Publications

A.P. Silva, J.O. Malva, A.F. Ambrósio, A.J. Salgado, A.P. Carvalho, C.M. Carvalho, “Role of Kainate Receptor Activation and Desensitization on the [Ca2+]i Changes in Cultured Rat Hippocampal Neurons,” Journal of Neuroscience Research, vol. 65, pp. 378-386, 2001.

A.J. Salgado, M.E. Gomes, A.Chou, O.P. Coutinho, R.L. Reis, D.W. Hutmacher, “Preliminary Study on the Adhesion and Proliferation of Human Osteoblasts on Starch Based Scaffolds,” Materials Science and Engineering C, vol. 20(1-2), pp. 27-33, 2002.

M. Endres, D.W. Hutmacher, A.J. Salgado, C. Kaps, J. Ringe, R.L. Reis, M. Sittinger, A. Brandwood, J.T. Schantz, “Osteogenic Induction of Human Bone Marrow Derived Mesenchymal Progenitor Cells in Novel Synthetic Polymer/Hydrogel Matrices,” Tissue Engineering, vol. 9(4), pp. 689-702, 2003.

E.T. Baran, K. Tuzlogoklu, A.J. Salgado, R.L. Reis, “Multichanel Mould Processing of 3D Structures from Microporous Coralline Hydroxyapaptite Granules and Chitosan Support Materials for Guided Tissue Regeneration/Engineering,” Journal of Materials Science: Materials in Medicine, vol. 15(2), pp. 161-165, 2004.

A.J. Salgado, O.P. Coutinho, R.L. Reis, “ Novel Starch Based Scaffolds for Bone Tissue Engineering: Cytotoxicity, Cell Culture and Protein Expression," Tissue Engineering, vol. 10(3-4), pp. 665-674, 2004.

A.J. Salgado, O.P. Coutinho, R.L. Reis, “Bone Tissue Engineering: State of the Art and Future Trends,” Macromolecular Bioscience, vol. s4, pp. 743-765, 2004.

A.J. Salgado, J.E. Figueiredo, O.P. Coutinho, R.L. Reis, “Biological Response to Pre-Mineralized Starch Based Scaffolds for Bone Tissue Engineering,” Journal of Materials Science: Materials in Medicine, vol. 16(3), pp. 267-275, 2005.

K. Tuzlakoglu, S. Egri, A.J. Salgado, M. E. Gomes, R.L. Reis, E. Piskin, “Nano and Micro-Fiber Combined Scaffolds: A new Architecture for Bone Tissue Engineering,” Journal of Materials Science: Materials in Medicine, 2005, in press.

A.J. Salgado, Y. Wang, J.F. Mano, R.L. Reis, “Influence of PLLA Molecular Weight and Cristalinity on the Adhesion and Proliferation of Human Osteoblast Like Cells,” Materials Science Forum, 2005, in press.

A.J. Salgado, O.P. Coutinho, R.L. Reis, J.E. Davies, “In Vivo Response to Starch Based Scaffolds for Bone Tissue Engineering Applications,” Journal of Biomedical Materials Research part A, 2005, accepted for publication.

A.J. Salgado, J.O. Oliveira, A. Pedro, R.L. Reis, “Adult Stem Cells for Bone and Cartilage Tissue Engineering,” Current Stem Cell Research and Therapy, 2005, submitted. Invited review paper

E.T. Baran, K. Tuzlakoglu, A.J. Salgado, R.L. Reis, “Micro channel patterned and heparin microcontact printed biodegradable membranes produced by compressive molding for tissue engineering applications,” Tissue Engineering, 2005, submitted.

Book Chapters

M.E. Gomes, A.J. Salgado, R.L. Reis, ”Bone Tissue Engineering Using Starch Based Scaffolds Obtained by Different Methodologies,” Polymer Based Systems on Tissue engineering, Replacement and Regeneration, ed.: R.L. Reis and D.Cohn, NATO/ASI Series, Kluwer Press, Drodercht, pp. 221-249, 2002. invited chapter

P.B. Malafaya, M.E. Gomes, A.J. Salgado, R.L. Reis, “Polymer Based Scaffolds and Carriers for Bioactive Agents from Different Natural Polymers,” Tissue Engineering, Stem Cells and Gene Therapies, ed.: Y.M. Elcin, Advances In Experimental Medicine and Biology Series, Kluwer Academic/Plenum Publishers, London, UK, pp. 201-233, 2003. invited chapter

A.J. Salgado, M.E. Gomes, O.P. Coutinho, R. L. Reis, “Isolation and Osteogenic Differentiation of Bone Marrow Progenitor Cells for Application in Tissue Engineering,” Biopolymer Methods in Tissue Engineering, ed.: A.P. Hollander, P.V. Hatton, Methods in Molecular Biology Series, The Humana Press Inc, Totowa, USA, pp. 123-130, 2003.

ME Gomes, AJ Salgado, RL Reis, “Inginiería de Tejidos: Principios Básicos, Soportes y Células Madre,” Biomateriales, Eds: R. Sastre, A. de Aza, J. San Roman, Faenza Editrice Iberica S.L, pp. 473-489, 2004.

A.J. Salgado, M.E. Gomes, O.P. Coutinho, R.L. Reis, “Tissue Engineering of Bone and Cartilage: The Biological Components,” Biodegradable Systems in Medical Functions: Design, Processing, Testing and Applications, ed.: R.L. Reis, J. San Román, CRC Press LLC, Florida, USA, pp. 457-478, 2004.

A.J. Salgado, M.E. Gomes, R.L. Reis, “Tissue Engineering of Mineralized Tissues: The Essential Elements,” Learning from Nature How to Design New Implantable Biomaterials: From Biomineralization Fundamentals to Biomimetic Materials and Processing Routes, ed.: RL Reis, S Weiner, NATO/ASI series, Kluwer Press, Drodercht, The Netherlands, pp. 205-222, 2004.

H.S. Azevedo, M.E. Gomes, P.B. Malafaya, A.P. Marques, A.J. Salgado, R.L. Reis, “Natural Origin Degradable Polymers in Biomedical Applications,” Handbook of Biodegradable Polymeric Materials and Their Applications, ed.: S.K. Mallapragada, B. Narasimhan, American Scientific Publishers, California, USA, Vol. 2, pp. 13-31.

Abstracts

A.J. Salgado, A.P. Silva, J.O. Malva, C.R. Oliveira., “Identification of Kainate Receptors in Cultured Hippocampal Neurons, Glial cells and Microglial cells,” XII National Conference of Biochemistry, Póvoa de Varzim, Portugal, 2000.

J.O. Malva, A.P. Silva, A.F. Ambrósio, A.J Salgado, C. Rego, C.R. Oliveira, “Profile of the Excitotoxic Action of AMPA and Kainate Receptor Activation in Cultured Hippocampal neurons: Role of Receptor Desensitization,” II Conference on Research in Medicine, Coimbra, Portugal, 2000.

J.O. Malva, P.C. Pinheiro, A.J. Salgado, R.A Cunha, C.R. Oliveira, “Kainate Receptor-Like Immunoreactivity in Macrophages and Microglia,” 15th Meeting of the Group on Studies in Ageing and Dementia, Peniche, Portugal, 2001.

A.J. Salgado, M.E. Gomes, R.L. Reis, D.W. Hutmacher, “Preliminary Study on the Adhesion and Proliferation of Human Osteoblasts on Starch Based Scaffolds,” International Conference on Materials for Avanced Technologies- ICMAT 2001, Singapore, Singapore, 2001.

A.J. Salgado, D.W. Hutmacher, H. Chin H, R.L. Reis, C.M. Agrawal, S.H. Teoh, “A Comparative Study Between Two Different Polycaprolactone (PCL) Scaffolds for Tissue Engineering,” International Conference on Materials for Advanced Technologies - ICMAT 2001, Singapore, Singapore, 2001.

M. Endres, J.T. Schantz, H.P. Too, A.J. Salgado, R.L. Reis, M. Sittinger, S.H. Teoh, D.W. Hutmacher, “Characterisation and Induction of Mesenchymal Stem Cells for Bone Reconstruction in Polymer Scaffolds,” International Conference on Materials for Advanced Technologies - ICMAT 2001, Singapore, Singapore, 2001.

A.J. Salgado, M.E. Gomes, O.P. Coutinho, R.L. Reis, D.W. Hutmacher, “Novel Starch Based Scaffolds for Tissue Engineering: Cell Culture and Protein Expression,” NATO/ASI Polymer Based Systems on Tissue engineering, Replacement and Regeneration, Alvor, Portugal, 2001.

M.E. Gomes, A.J. Salgado, J.S. Godinho, A.M. Cunha, R.L. Reis, "Manufacturing and In Vitro Biocompatibility of Starch Based Degradable Scaffolds for Bone Tissue Engineering," 1st Biennial Meeting of the European Tissue Engineering Society/4th International Meeting of the Tissue Engineering Society International, Freiburg, Germany, 2001.

A.J. Salgado, A.L. Oliveira, M.E. Gomes, O.P. Coutinho, R.L. Reis, "Evaluating Several Biomimetic Coating Routes Aimed at Improve the Cellular Adhesion to Starch Based Scaffolds," 1st Biennial Meeting of the European Tissue Engineering Society/4th International Meeting of the Tissue Engineering Society International, Freiburg, Germany, 2001.

A.J. Salgado, M.E. Gomes, O.P. Coutinho, R.L. Reis, D.W. Hutmacher, “A Qualitative Study on the Proliferation of Human Osteoblasts Cultured on Novel Starch Based Tissue Engineering Scaffolds,” 1st Biennial Meeting of the Tissue Engineering Society/4th International Meeting of the Tissue Engineering Society International, Freiburg, Germany, 2001.

M. Endres, J.T. Schantz, A.J. Salgado, M. Sittinger, R.L. Reis, S.H. Teoh, D. W. Hutmacher, “Osteogenic Induction of Human Osteoprogenitor Cells in Polycaprolactone Based Scaffolds,” 1st Biennial Meeting of the Tissue Engineering Society/4th International Meeting of the Tissue Engineering Society International, Freiburg, Germany, 2001.

D. W, Hutmacher, M. Endres, J. T. Schantz, H. P. Too, A. J. Salgado, R. L. Reis, M. Sittinger, S. B. Ismail, “Characterisation and Induction of Mesenchymal Stem Cells in Polymer Scaffolds,” 5th Asian Symposium on Biomedical Materials, Hong Kong, China, 2001.

M.E. Gomes, A.J. Salgado, J.S. Godinho, A.M. Cunha, D.W. Hutmacher, R.L. Reis, “Starch Based Scaffolds for the Tissue Engineering of bone: Mechanical Properties and Biological Response to Osteoblastic Cells,” 28th Annual Meeting of Society for Biomaterials, Tampa Bay, USA, 2002.

A.J. Salgado, A.L. Oliveira, O.P. Coutinho, R.L. Reis, “Evaluating Biomimetic Ca-P Coatings for Improvement of Cellular Adhesion to Starch Based Scaffolds,” 17th Conference of the European Society for Biomaterials, Barcelona, Spain, 2002.

M.E. Gomes, A.J. Salgado, J.S. Godinho, A.M. Cunha, D.W. Hutmacher, R.L. Reis, "Evaluation of the Mechanical Properties and Biological Response to Osteoblastic Cells of Starch Based Scaffolds for Bone Tissue Engineering Applications," 17th Conference of the European Society for Biomaterials, Barcelona, Spain, 2002.

A.J. Salgado, J.E. Figueiredo, O.P. Coutinho, R.L. Reis, “Biological Effects of the Pre-Mineralization of Starch Based Scaffolds for Bone Tissue Engineering,” 5th International Meeting of the Tissue Engineering Society International, Kobe, Japan, 2002.

E.T. Baran, K. Tozlakoglu, A.J. Salgado, R.L. Reis, “Processing of 3D Architectures Based on HA Porous Particles/Chitosan Fibres/Chitosan Membranes For Guided Tissue Regeneration Using a Multichannel Mold,” 5th International Meeting of the Tissue Engineering Society International, Kobe, Japan, 2002.

A.J. Salgado, Ana L. Oliveira, J.E. Figueiredo, O.P. Coutinho, R.L. Reis, “Biological Response to 3D Pre-Mineralized Scaffolds for Bone Tissue Engineering: Cytotoxicity, Cell Culture and Protein Expression,” 29th Annual Meeting of the Society for Biomaterials, Reno, USA, 2003.

K. Tuzlakoglu, C. M. Alves, A. J. Salgado, J. F. Mano, R. L. Reis, “Development, Dynamical-Mechanical Properties and Biological Performance of Novel 3-D Chitosan Fiber Mesh Scaffolds,” 29th Annual Meeting of the Society for Biomaterials, Reno, USA, 2003.

A.J. Salgado, O.P. Coutinho, Rui L. Reis, J.E. Davies, “Preliminary Study on the In Vivo Response to Starch Based Scaffolds for Bone Tissue Engineering Applications,” 23rd Annual Meeting of the Canadian Biomaterials Society, Montreal, Canada, 2003.

A.J. Salgado, O.P. Coutinho, R.L. Reis, “Novel Starch Based Polymers: A Promissing Alternative for Bone Tissue Engineering Applications,” 2nd Annual Meeting of the European Tissue Engineering Society, Génova, Italy, 2003.

A.J. Salgado, O.P. Coutinho, D.W. Hutmacher, J.E. Davies, R.L. Reis, “In Vitro and In Vivo Evaluation of the Potential of Starch Based Scaffolds for Bone Tissue Engineering Scaffolding,” NATO/ASI Learning from Nature How to Design New Implantable Biomaterials: From Biomineralization Fundamentals to Biomimetic Materials and Processing Routes, Alvor, Portugal, 2003.

K. Tuzlagoklu, E.T. Baran, A.J. Salgado, O.P. Coutinho, R.L. Reis, “In Vitro Evaluation of Microporous Coralline-Chitosan Composite Materials for Bone Tissue Engineering,” NATO/ASI Learning from Nature How to Design New Implantable Biomaterials: From Biomineralization Fundamentals to Biomimetic Materials and Processing Routes, Alvor, Portugal, 2003.

A.J. Salgado, O.P. Coutinho, R.L. Reis, J.E. Davies, “In Vivo Response to Starch Based Scaffolds for Bone Tissue Engineering Applications,” 6th International Meeting of the Tissue Engineering Society International (TESI), Orlando, USA, 2003.

A.J. Salgado, O.P. Coutinho, D.W. Hutmacher, J.E. Davies, R.L. Reis, “Extruded Starch Based Scaffolds for Bone Tissue Engineering,” XVIIth Aachen Colloquium on Biomaterials, Aachen, Germany, 2004.

A.J. Salgado, O.P. Coutinho, R.L. Reis, J.E Davies, “In Vivo Evaluation of Extruded Starch Based Scaffolds for Bone Tissue Engineering Applications,” 7th World Biomaterials Congress, Sydney, Australia, 2004.

E.T. Baran, K. Tuzlagoklu, A.J. Salgado, R.L. Reis, "Production of Micro-Capillary Patterned Lamellar Scaffolds for Tissue Engineering by Using Pressure Micro Moulding Imprinting Technology," 7th World Biomaterials Congress, Sydney, Australia, 2004.

A. Pedro, E.T. Baran, A.J. Salgado, R.L. Reis, “Adhesion and Proliferation of Osteoblastic Like Cells on Collagen Coated Starch Based Scaffolds for Bone Tissue engineering Applications,” 7th International Meeting of the Tissue Engineering Society International (TESI), Lausagne, Switzerland, 2004.

A.J. Salgado, D.W. Hutmacher, J.E. Davies, R.L. Reis, “Starch/Cellulose Acetate and its Adequatibility to be Used as Scaffolds for Bone Tissue Engineering,” 51st Meeting of the Orthopaedic Research Society (ORS), Washington D.C., USA, 2005.

A.J. Salgado, O.P. Coutinho, D.W. Hutmacher, J.E. Davies, R.L. Reis, “Starch Based Scaffolds Designed for Bone Tissue Engineering Applications: Cytotoxicity, Cell Culture and In Vivo Evaluation,” 30th Annual Meeting of the Society for Biomaterials, Memphis, USA, 2005.

J.T. Oliveira, A.J. Salgado, V.M. Correlo, A.R. Pinto, M. Battacharya, P. Charbord, N.M. Neves, R.L. Reis, “Preliminary Assessement of the Potential of Chitosan/Polyester Based Scaffolds for Bone and Cartilage Tissue Engineering,” 30th Annual Meeting of the Society for Biomaterials, Memphis, USA, 2005.

A.J. Salgado, Y. Wang, J.F. Mano, R.L. Reis, “Influence of PLLA Molecular Weight and Cristalinity on the Adhesion and Proliferation of Human Osteoblast Like Cells,” XII Portuguese Materials Society Meeting, Aveiro, Portugal, 2005.

J.T. Oliveira, A.J. Salgado, V.M. Correlo, A.R. Pinto, M. Bhattacharya, P. Charbord, N.M. Neves, R.L. Reis, “Preliminary Assessment of the Behaviour of Chitosan/Poly(Butylene Succinate) Scaffolds Seeded with Mouse Mesenchymal Stem Cells for Cartilage Tissue Engineering," 19th Conference of the European Society for Biomaterials, Sorrento, Italia, 2005.

A.R. Pinto, A.J. Salgado, J.T. Oliveira, V.M. Correlo, M. Bhattacharya, P. Charbord, R.L. Reis, N.M.Neves, “Evaluation of the Adhesion, Proliferation and Differentiation of a Mouse Mesenchymal Stem Cell line on Novel Chitosan/Polyester Based Scaffolds,” 19th Conference of the European Society for Biomaterials, Sorrento, Italia, 2005.

K. Tuzlakoglu, S. Egri, A.J. Salgado, M. E. Gomes, E. Piskin, R.L. Reis, “Nano- and Micro-fiber Combined Scaffolds: A New Architecture for Bone Tissue Engineering,” 19th Conference of the European Society for Biomaterials, Sorrento, Italia, 2005.

K.Tuzlakoglu, A.J.Salgado, R.L. Reis,"Development of Silk fibroin-coated Chitosan Fiber Mesh Scaffolds for Bone Tissue Engineering," 6th International Symposium on Frontiers in Biomedical Polymers, Granada, Spain, 2005.

K. Tuzlakoglu, A.J. Salgado, R.L. Reis,"Formation of Ca-P Layer on Chitosan Fiber Mesh Scaffolds by a Biomimetic Process and Its Effect on Osteoblast Cell Morphology and Viability," European Congress on Advanced Materials and Processes (Euromat 2005), Prague, Czech Republic, 2005.

T.Inoue, S.R.Smukler, T.Holowacz, Y.Inoue, A.J.Salgado, W.Akamatsu, Y.Tano, D. Van der Kooy, “Concurrent OTX2 and PAX6 Overexpression Accompanied by retinoic Acid Exposure Induces Eye Field identity in ES-derived Primitive Neural Stem Cells,” 35th Annual Meeting of the Society for Neurosciences, Washington D.C., USA, 2005.

A.M. Martins, A.J. Salgado, H.S. Azevedo, I.B. Leonor, R.L. Reis, “Lysozyme incorporation in biomimetic coated chitosan scaffolds: development and behaviour in contact with osteoblastic-like cells,” 8th Annual Meeting of the Tissue Engineering Society International (TESI), Shangai, China, 2005, accepted-podium.

Other Publications

Front Cover Photograph: “ Novel Starch Based Scaffolds for Bone Tissue Engineering: Cytotoxicity, Cell Culture and Protein Expression “, Tissue Engineering, vol.10(3-4), pp. 665-674, 2004.

PhD Thesis, “Biological Assessment of Melt Processed Starch based Scaffolds for Bone Tissue Engineering Applications”, University of Minho, 2005.